1 min read
Evaluating Corneal Damage from 222-nm and 254-nm UV Light in Rats
UV Medico
:
Mar 5, 2024 10:09:02 AM

222nm ultraviolet (UV) light produced by a krypton–chlorine excimer lamp is harmful to bacterial cells but not to skin. However, the effects of 222-nm UV light exposure on the eye are not fully known. We evaluated acute corneal damage induced by 222- and 254-nm UV light in albino rats. Under deep anesthesia, 6-week-old Sprague–Dawley albino rats were exposed to UV light.
The exposure levels of corneal radiation were 30, 150, and 600 mJ/cm2. Epithelial defects were detected by staining with fluorescein. Superficial punctate keratitis developed in corneas exposed to more than 150mJ/cm2 of UV light, and erosion was observed in corneas exposed to 600mJ/cm2 of UV light. Haematoxylin and eosin staining also showed corneal epithelial defects in eyes exposed to 254-nm UV light. However, no damage developed in corneas exposed to 222-nm UV light. Cyclobutane pyrimidine dimer-positive cells were observed only in normal corneas and those exposed to 254-nm UV light.
Although some epithelial cells were stained weakly in normal corneas, squamous epithelial cells were stained moderately, and the epithelial layer that was detached from the cornea exposed to 600mJ/cm2 of light was stained intensely in corneas exposed to 254-nm UV light.
In the current study, no corneal damage was induced by 222-nm UV light, which suggested that 222-nm UV light may not harm rat eyes within the energy range and may be useful for sterilising or preventing infection in the future.
Latest knowledge from UV Medico

Far-UVC - Effectively Disinfects SARS-CoV-2 Surface Contamination


Far-UVC: Significantly Safer for Eyes, Proven by Corneal Damage Study


Ultraviolet Lighting During Orthopaedic Surgery and Rate of Infection


Harmless Effects of 222 nm Far-UVC Radiation on Mouse Skin and Eyes


Long-Term Study: UVC Lamps Safe for Mice Sensitive to UV Radiation


1 min read
Safe Long-Term Use of 222 nm UVC Lamps on UV-Sensitive Mice
