Micro CRISPR

Case Study · Micro CRISPR

Can far-UVC cut cleanroom bioburden during operation?

Challenge

Microbial control in a cleanroom is continuous — contamination keeps returning through personnel, airflow, and routine operations.

Approach

A GMP-aligned, three-phase (A-B-A) settle-plate demonstration of 222 nm Far-UVC during active cleanroom operation.

Result

62% fewer bacteria (42 → 16 CFU) and fungal contamination to zero — within Grade C limits, no ozone excursions.

The Challenge

Contamination keeps coming back

Maintaining microbial control in cleanroom and controlled environments is a continuous challenge.

Cleaning and disinfection are essential, yet contamination pressure reoccurs through personnel activity, airflow dynamics, and routine operations. Micro CRISPR set out to test whether continuous UV222 exposure could measurably reduce bioburden during active operation — while keeping conditions safe and compliant.

The Approach

A GMP-aligned A-B-A demonstration

Micro CRISPR ran an in-room demonstration of 222 nm Far-UVC (UV222) at its facility.

The study used settle plates at critical locations — an agar surface exposed for four hours so airborne bacteria and fungi deposit, then incubated and counted as colony-forming units (CFU). It followed a three-phase protocol with triplicate samples in each phase: Phase 1 baseline (UV off), Phase 2 UV222 active (UV on), Phase 3 return to baseline (UV off).
UV222 Cleanroom Downlight
The UV222 Cleanroom Downlight, installed in-room for the demonstration.

The Result

62% fewer bacteria. Fungal to zero.

During UV222 operation, bacterial counts fell from 42 CFU to 16 CFU, and fungal contamination from 5 CFU to 0.

The results were consistent with Grade C environmental limits and were achieved during normal occupancy — not an unoccupied terminal cycle. Ozone was monitored in the breathing zone with no excursions, no visible material degradation was observed, and sampling followed the site’s standard operating procedure.
“Measurable bioburden reduction during active operation — safe, continuous, and chemical-free.”

Want the full data?

The full demonstration report with methodology and results can be linked here.

#">Read the full report

Far-UVC for your cleanroom?

Talk to our team about UV222 for GMP and controlled environments. Full references available on request.

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